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FLS 2010 Workshop Program: Science with next-generation hard X-ray sources – S. Wakatsuki (KEK)

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Title

Science with next-generation hard X-ray sources – S. Wakatsuki (KEK)

Location

Panofsky Auditorium

Start Time

3/2/2010 9:00 AM

End Time

3/2/2010 9:30 AM

Abstract

Science with Future Hard X-ray Sources: Exploration of Protein Universe

Soichi Wakatsuki
Photon Factory, Structural Biology Research Center
Institute of Molecular Structure Science, KEK, Tsukuba, Japan
 
Synchrotron radiation provides intense, tuneable and very parallel X-ray beams suitable for structural investigations of proteins and their complexes, their atomic structures, dynamics, and higher order architecture of organelles, cells, and tissues. Future light sources are expected to expand these research areas even further leading to a new level of understanding of machinery of life with quantum leaps in some areas. Future directions of the four critical synchrotron techniques, X-ray protein crystallography, scattering, spectroscopy, and imaging will be discussed with needs from biology using our recent research activities in protein transport, membrane remodelling and ubiquitin signalling.
 Upgrading storage ring based synchrotron facilities aims to provide lower emittance, higher brilliance X-ray beams which will enable protein crystallography to its extreme, i.e., nano crystals, extremely large complexes, as well as the highest efficiency in structure determination of proteins and their complexes. Protein complexes are very often fragile, dynamic and transient (i.e., change partners as they go through different stages of function), hence difficult to crystallize. Solution X-ray scattering provides an ideal, complementary technique by providing medium to low resolution structures in solution. In future, exploitation of anomalous signals in solution scattering will bring in important additional information on sub-molecular distances from the labels incorporated in the complexes for understanding of their structure and dynamics. At the higher end of the hierarchical understanding of molecular cell biology, cell/organelle imaging (bio nanoproble) will provide critical understanding of higher order structures combined with higher resolution structures of the components and spectroscopic information. ERL will not only push these even further with much higher brightness and capacity, but also enable time resolved diffraction, scattering and spectroscopy experiments with shorter than 1 ps resolution and high repetition rate. In the X-ray FEL development, single molecule structure determination using coherent diffraction is one of the most challenging projects in structural biology research, aiming at determination of protein (complex) structures at 2 to 4 Angstrom resolution from diffraction images of single molecules. These methods are all required to cope with the future needs in investigation of the rapidly growing protein universe: vast interaction networks of biological molecules, metagenomics with several million new protein sequences, non-coding RNAs, and human microbiomes. Finally, it will be essential that these synchrotron techniques be combined with other structural biology methods such as electron microscopy and tomography, NMR, optical live cell imaging, neutron diffraction/scattering/reflectivity, and other physical and informatics methods to complement the synchrotron molecular cell biology research.

All Day Event

 

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Key

Plenary sessions

Color

#996600

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<div style='border: 1px solid #996600; padding: 5px; margin: 0px; color: #996600;background-color: #ffff99;'>Science with next-generation hard X-ray sources – S. Wakatsuki (KEK)</div>

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Attachments

2010-03-02_ICFA_FLS_Wakatsuki_for_WEB.pdf    
Content Type: Event
Version: 8.0
Created at 2/1/2010 2:01 PM by Chan, Andrea
Last modified at 8/31/2010 10:38 AM by ykotturi

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