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An Office of Science User Facility

Macromolecular Femtosecond Crystallography (MFX)

 

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MFX Standard Configuration #1 for Run 16

Types of Experiments

With this standard configuration, MFX will be able to support fixed target Serial Femtosecond Crystallography (SFX) experiments at atmospheric pressure and at room temperature or cryogenic temperatures (278 to 100K). A controlled humidity stream (30.0 % to 99.8 % RH) is available. The system is highly automated for rapid sample exchange and sample positioning. The detector will be a Rayonix 325 MX provided by SMB (1 Hz readout).

X-ray Focus

The incident photon energy is preferred to be 9.5 keV but justified deviations can be considered. The x-ray focus can vary over a range of ~2-3 µm to ~100 µm.

Sample delivery

A goniometer system developed by the Structural Molecular Biology (SMB) team at SSRL will be deployed in the MFX hutch for this purpose. The system consists of a goniometer with translation and rotation capabilities to precisely orient and scan crystals, and an on-axis (visible and NIR light compatible) sample microscope. The crystals can be mounted on grids, chips, or loops, meshes or any standard mounting system already supported by the SMB group.

Measurements at cryo-cooled temperatures or room temperature will be possible.

The setup includes the option of an on-axis cryo-cooler to maintain crystals at temperatures of 278 to 100K during the measurements. An Arinax humidity control instrument is available for controlled humidity (30.0 % to 99.8 % RH) during measurements at ambient temperatures.

A sample exchange robot, the Stanford Automated Mounting (SAM) system, may be used to exchange samples on compatible magnetic bases held in SSRL cassettes or uni-pucks from inside an LN2 liquid nitrogen filled storage Dewar: http://smb.slac.stanford.edu/users_guide/manual/Using_SSRL_Automated_Mounti.html. Samples at ambient temperatures may be mounted from 10 sample base storage locations on the side of the SAM robot.

More detailed information on what is supported by the standard SMB suite of capabilities for sample mounting, data collection modes, etc. can be found here. Please contact SMB support staff prior to the experiment for specific information on sample compatibility and size restrictions, borrowing SAM compatible cryotools and sample holders, or the availability of custom automation in sample centering and positioning. For users new to the SAM robot, a kit of supplies for using the SAM robot for sample exchange with cryogenically cooled samples may be lent to your group prior to the experiment (to be returned after the experiment).

Detector

The detector will be a Rayonix 325 MX (1 Hz readout) provided by SMB.

Control System

The data collection will be performed using Blu-Ice/DCSS .

SMB Staff Supporting Goniometer Operations at LCLS

Aina Cohen (main contact), Mike Soltis, Clyde Smith, Silvia Russi, Ana Gonzalez, Artem Lyubimov, Jinhu Song, Scott McPhillips, Paul Ehrensberger and Mike Hollenbeck.

MFX Instrument Staff

Alex Batyuk, Sergio Carbajo, Andy Aquila, Mark Hunter, TJ Lane, Meng Liang, Matt Seaberg, Ray Sierra, Sebastien Boutet

Required Acknowledgment

Use of this Standard Configuration is contingent on acceptance by the users to use the following acknowledgment in any presentation or publication: "Use of the Linac Coherent Light Source (LCLS) and Stanford Synchrotron Radiation Lightsource (SSRL), SLAC National Accelerator Laboratory, is supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Contract No. DE-AC02-76SF00515. The SSRL Structural Molecular Biology Program is supported by the DOE Office of Biological and Environmental Research, and by the National Institutes of Health, National Institute of General Medical Sciences (including P41GM103393). The contents of this publication are solely the responsibility of the authors and do not necessarily represent the official views of NIGMS or NIH."

Parameter Table

To be considered for scheduling in this standard configuration, users will be required to include a table in the proposal that lists the specific experimental parameters to ensure compatibility with these configurations. If the experimental parameters are not compatible with the standard configuration or if the table of parameters is incomplete, the proposal will be reviewed and considered for scheduling as general user proposal. Please see the table of required parameters. No fundamental changes to the standard configurations will occur, but some details of the configuration may be updated in response to inquiries, so users should recheck the website before submitting your proposal to confirm that you have the latest information. Address any questions to the instrument staff.
 
 

LCLS proposals are submitted through the User Portal.


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MFX Standard Configuration #2 for Run 16

Types of Experiments

With this standard configuration, MFX will be able to support jet-based Serial Femtosecond Crystallography (SFX) experiments at atmospheric pressure and temperature with noise minimization from the Helium environment. This will be possible with or without a pump laser. Also supported will be Small Angle and Wide Angle X-ray Scattering (SAXS/WAXS) with or without a pump laser using the wide variety of sample delivery jets, either LCLS-owned or supplied by the user groups. The detector will be a Rayonix 170 capable of 10 Hz operation with 1920x1920 pixels.

X-ray Focus

The incident photon energy is preferred to be 9.5 keV but justified deviations can be considered. The x-ray focus can vary over a range of ~2-3 µm to ~100 µm.

Sample Environment

The Helium-Rich Ambient (HERA) instrument will be used. It provides a helium filled volume for the sample and the detector, including the following features that will be supported in this standard configuration.

Liquid Jet Mechanical System

The liquid jet mechanical system consists of an XYZ stage to position a nozzle inside the helium box. Any sample delivery system which is compatible with this mechanical system and the standard nozzle mount will be supported. This included Gas Dynamic Virtual Nozzle (GDVN), Lipidic Cubic Phase (LCP), the Microfluidic Electrokinetic Sample Holder (MESH) or other viscous extrusion systems, many types of mixing nozzles and any other system that can be mounted on the nozzle mount. Contact the MFX scientists for details on the nozzle rod and the injector mechanical system.

On-axis Sample Viewing System

The HERA system at MFX will be equipped with an on-axis jet viewing system. This system provides a ~3 micron resolution view of the jet from the perspective of what the x-ray beam sees. This provides a rough alignment of the jet without any detailed information about the sample content or jet size and speed.

Perpendicular High Resolution Jet Imaging System

A high resolution jet imaging system will be installed to provide a perpendicular view of the jet. A continuous illumination will be used to provide the necessary illumination.

Optical Pump Lasers

Time-resolved experiments employing tunable nanosecond pulses will be supported under this standard configuration.

Nanosecond laser

An Opolette HR 355 will be available for <8 ns pump laser pulses over a wide tunability range (410-2200 nm). This laser will be fiber-coupled then propagated in free-space into the sample chamber and combined collinearly with the x-ray, with in-coupling ~250 mm upstream of the sample and with the focusing lens ~350mm away from the sample. Note that the efficiency of the optics and the performance of the laser will not be the same for the entire wavelength range. The maximum achievable power density will be determined by the existing optical system and will not be modified. Contact the MFX scientists to discuss specific needs and determine if they can be achieved using this standard configuration of the laser. The expected performance at the source is shown below (the energy on target will be substantially lower due to transport losses). The MFX system will have the following options:



Timing diagnostics

For the nanosecond laser, a fast diode will be available to verify the time overlap of the laser and the x-rays.

Detector

The detector will be a Rayonix 170 capable of 10 Hz operation with 1920x1920 pixels. Other binning settings can increase the repetition rate.

MFX Instrument Staff

Alex Batyuk, Sergio Carbajo, Andy Aquila, Mark Hunter, TJ Lane, Meng Liang, Matt Seaberg, Ray Sierra, Sebastien Boutet

Required Acknowledgment

Use of this Standard Configuration is contingent on acceptance by the users to use the following acknowledgment in any presentation or publication: "The HERA system for in helium experiments at MFX was developed by Bruce Doak and funded by the Max-Planck Institute for Medical Research."

Parameter Table

To be considered for scheduling in this standard configuration, users will be required to include a table in the proposal that lists the specific experimental parameters to ensure compatibility with these configurations. If the experimental parameters are not compatible with the standard configuration or if the table of parameters is incomplete, the proposal will be reviewed and considered for scheduling as general user proposal. Please see the table of required parameters. No fundamental changes to the standard configurations will occur, but some details of the configuration may be updated in response to inquiries, so users should recheck the website before submitting your proposal to confirm that you have the latest information. Address any questions to the instrument staff.
 
 
 

LCLS proposals are submitted through the User Portal.

 

 

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